This AP Biology level lab is designed to be equivalent to a college level lab. This lab is a student-driven discovery process based on a real-world Shigella outbreak in the year 2000. Students use scientific reasoning to mimic a foodborne outbreak investigation and design an experiment using gel electrophoresis to determine the source of the outbreak.
The intent of this lab exercise is to engage and encourage students to ask the right questions and develop the skill sets need to approach a problem.
- Translate information expressed as text into other visual information forms, such as tables or charts, in order to systematically analyze data
- Apply the principles of experimental design to develop a logical experiment and test a hypothesis
- Develop an understanding of foodborne illnesses and foodborne outbreaks
- Practice logical deduction to determine a probable conclusion
- Practice experimental design
- Understand DNA analysis
- Kit components: 10 1% Agarose GreenGel in-a-Cup, 100 ml MiniOne TBE Concentrate, 11 DNA samples, pipette tips, microcentrifuge tubes, a teacher guide & student worksheet
- Supplies and materials enough for 10 runs
- Store at 4°C refrigerator (especially for the DNA samples); GreenGel-in-a-Cup should be protected from light
- Guarantee stable for three months with proper storage