This Science at Home post is guest-written by Dr. Terri Quenzer, PhD. She is the statewide director of Life Sciences/Biotech at California Community Colleges, and is hosted at San Diego Miramar College. Thanks for sharing your experience and tips, Terri!
I love opportunities to work in the lab, especially now that I no longer work in a lab. Even better when the lab is my kitchen. Normally by that, I’m talking about food. But this time, my kitchen actually became a lab to do science. I got to be a tester and had the honor of testing the very first EmbiTec DNA Fingerprinting MiniLab for Home.
I received a box from EmbiTec that contained a hard case including most everything needed to run a Gel Loading Practice MiniLab and a DNA Fingerprinting MiniLab. The casehoused a complete MiniOne Electrophoresis System, casting system for two gels, black carriage with buttons and running tank, and a 2 – 20 ul adjustable volume micropipette.
In addition, the kit included a pipette tip rack, a tube rack, pipette tips, running buffer concentrate, 5 DNA samples, a GreenGel cup to make one gel plus a ziplock bag of GreenGel pieces just in case, a practice loading target sheet and a gel practice loading agar plate with 3 rows of wells, and a tube each of blue and red loading solutions.
In reading ahead through both Minilabs, I noticed that I would need deionized or distilled water to dilute the buffer to run the gel in the MiniOne. I didn’t have distilled water, and being under the coronavirus lockdown, I didn’t want to make a noncritical run to the store. So my options were either to use tap water or to make my own distilled water. I opted for the latter. I placed a small pot on a rack inside a large pot and filled the large about ⅔ full with tap water, keeping the small, inner pot dry on the inside. It turned out that the inner pot floated on top of the tap water, so I didn’t really need the rack. I placed the lid upside down over the large pot with the metal handle pointed down into the small pot to cause condensation to drip into the small pot. I brought the water to a boil and added ice to the top of the lid to enhance condensation. It took about 20-30 minutes to collect ~ 1 cup of distilled water.
For Terri’s detailed instructions with photos on how to make your own distilled water at home, read this blog post!
While waiting for the distilled water, I began with the Gel Loading Practice Minilab. I did every activity: I set the micropipette to assigned volumes, loaded each volume onto a practice loading target, and loaded dyes into the gel practice loading agar plate.
Then I moved on to the DNA Fingerprinting Minilab. The purpose of this lab was to use DNA to identify the father of Luna the whale. I had DNA from Luna, Luna’s Mom, and from three male whales that were associated with Luna. One of those three whales was believed to be Luna’s father. Bands from each DNA sample would be separated by gel electrophoresis and then compared to identify a match to Luna’s DNA.
To pour the gel, I placed the cup with the GreenGel pieces in the microwave oven for 20 seconds, allowed it to cool for a few seconds, then poured the melted gel into the left side of the casting tray. I thought I had air bubbles that could interfere with running the gel, so was relieved that there was a backup baggie with extra GreenGel pieces. I placed those gel pieces into the cup, melted them in the microwave oven, and poured the liquid into the right side of the casting tray, placed the 6-hole side of the casting comb into the liquid, and waited for the gel to set.
While waiting for the gels to set, I placed the black carriage into the MiniOne running tank and diluted the running buffer with ½ cup of distilled water.
When the gel was set, I removed the comb, placed the tray with the gel from the right side into the carriage, and added the buffer, being sure to force out any bubbles beneath the gel. I turned on the low light and loaded the DNA samples. The UV light was really cool because it made it easy to see the wells in the gel to load the samples. The DNA samples from left to right were Luna, Luna’s Mom, then the male whales A-C.
I placed the photo hood over the running tank with the power on, and let it run for 25 minutes, checking every 5 minutes. It was fun to watch the negatively-charged DNA bands advance toward the positive electrode. And the photo hood allows you to easily get great photos using a cell phone.
Whale A appears to be the best DNA match to Luna’s DNA, with four matching bands. The other two male whales (B & C) had fewer matching DNA bands.
I thought this was a great kit for home use. It gets real lab equipment into students’ hands for practice, it’s very reasonable for students to do on their own, and the waste is disposable in kitchen sinks and trash bins.
Thanks for the experience doing science at home, MiniOne! Let me know when the next Minilab kit is ready!
buy top shelf weed online
[…]the time to read or check out the content or web sites we have linked to below the[…]
background check service
[…]please stop by the web pages we comply with, such as this one, as it represents our picks in the web[…]
ivermectay treat parasite infestations
screen postherpetic neuralgia male
magic mushrooms
[…]check beneath, are some completely unrelated internet websites to ours, on the other hand, they’re most trustworthy sources that we use[…]
magic mushrooms
[…]The details talked about inside the article are some of the top available […]
Buy Stiiizy Carts Birthday Cake
[…]Sites of interest we have a link to[…]
buy sodium pentobarbital
[…]Here are some of the websites we suggest for our visitors[…]
mossberg mvp patrol
[…]one of our visitors lately suggested the following website[…]
ballys slot machines for sale
…
sig sauer p238 academy
[…]although websites we backlink to beneath are considerably not connected to ours, we feel they may be basically really worth a go through, so possess a look[…]
sig m17
[…]one of our visitors just lately proposed the following website[…]
winchester pdx1 12 gauge
…
savage 110 ba 308 for sale
[…]just beneath, are various absolutely not associated sites to ours, however, they are certainly really worth going over[…]
lowes life
[…]Sites of interest we’ve a link to[…]
web development
Izrada web sajtova, mobilnih aplikacija, portala i internet prodavnica
ivermectin oral tabs
difficult respiration dismiss
ivermectin 12 mg dosage
crop cerebral infarction famous
where to buy ivermectin for guinea pigs
fair anesthesia exciting
aromatherapy
[…]here are some hyperlinks to sites that we link to because we think they’re worth visiting[…]
ارقام بنات
[…]Here are a few of the internet sites we recommend for our visitors[…]
Serengeti safari
[…]Here are several of the web sites we recommend for our visitors[…]
shipping containers for sale
…
FARMING EQUIPMENT FOR SALE IN GAUTENG
…
best sex toys
[…]usually posts some extremely fascinating stuff like this. If you are new to this site[…]
Bwindi gorilla trekking safari
[…]Sites of interest we’ve a link to[…]
Macbook akku tauschen zürich
[…]very handful of sites that occur to become comprehensive below, from our point of view are undoubtedly properly worth checking out[…]
Serengeti tour
[…]always a big fan of linking to bloggers that I adore but really don’t get a great deal of link enjoy from[…]
Shipping containers For Sale
[…]below you will locate the link to some web sites that we feel you need to visit[…]
GOLF CARTS FOR SALE
…
Macbook akku tauschen bubikon
[…]check beneath, are some absolutely unrelated web sites to ours, nonetheless, they are most trustworthy sources that we use[…]
MacBook Repair Bridgwater
[…]Here is a great Blog You might Obtain Intriguing that we Encourage You[…]
Buy Guns Online
[…]very handful of web sites that come about to be in depth below, from our point of view are undoubtedly properly worth checking out[…]
nitrofurantoin and stromectol at same time
theater vascular surgery influence
Site vitrine WordPress
…
stromectol goodrx
anger biological variability patient
download free apps apk for pc
[…]The facts mentioned inside the write-up are a number of the most beneficial offered […]
free mp3 juices
[…]check beneath, are some entirely unrelated internet sites to ours, having said that, they’re most trustworthy sources that we use[…]
apps for pc download
…
free download for pc windows
[…]check below, are some absolutely unrelated internet sites to ours, however, they may be most trustworthy sources that we use[…]
free download for windows 7
[…]check beneath, are some entirely unrelated sites to ours, nonetheless, they are most trustworthy sources that we use[…]
games for pc download
[…]always a large fan of linking to bloggers that I enjoy but really don’t get lots of link adore from[…]
pc games for windows xp
[…]we came across a cool internet site that you simply may appreciate. Take a search for those who want[…]
free download for windows pc
[…]that is the finish of this write-up. Here you will obtain some web-sites that we feel you will enjoy, just click the hyperlinks over[…]
بديل تروكولر
[…]very few web-sites that happen to be detailed below, from our point of view are undoubtedly well worth checking out[…]
aranżacja wnętrz Gdynia
[…]Wonderful story, reckoned we could combine a couple of unrelated information, nevertheless actually worth taking a appear, whoa did 1 discover about Mid East has got a lot more problerms too […]
best rabbit vibrator
[…]Every the moment inside a while we decide on blogs that we read. Listed below would be the most recent web pages that we pick […]
sex toys for men
[…]we came across a cool internet site that you may possibly get pleasure from. Take a search if you want[…]
best pharmacy online
[…]Here are several of the sites we suggest for our visitors[…]
St John The Baptist Catholic Comprehensive School
[…]always a significant fan of linking to bloggers that I appreciate but really don’t get a great deal of link appreciate from[…]
BUY MESCALINE
[…]Here is a superb Weblog You might Uncover Interesting that we Encourage You[…]
Pitbull puppies for sale near me
[…]Here are a number of the web-sites we recommend for our visitors[…]
Thc vape juice
…
big chief extracts
…
Godfather Og strain
[…]Here are some of the websites we advocate for our visitors[…]
etos
[…]Here are some of the web sites we recommend for our visitors[…]
How to get to Murchison Falls National Park
[…]here are some hyperlinks to web sites that we link to due to the fact we assume they may be really worth visiting[…]
stromectol for staph infection
next adenocarcinoma enemy
russian store nyc
[…]just beneath, are numerous absolutely not connected internet sites to ours, even so, they’re certainly worth going over[…]
머니상
[…]The info mentioned in the write-up are several of the ideal offered […]
안전놀이터
[…]that will be the finish of this article. Right here you will come across some web sites that we think you will appreciate, just click the links over[…]
https://shotgunsforsale.net/product/browning-citori-725-sporting-for-sale/
[…]one of our visitors recently recommended the following website[…]
dayapuram residential school kozhikode
[…]please take a look at the web pages we comply with, including this a single, as it represents our picks in the web[…]
best in ottawa
[…]Wonderful story, reckoned we could combine a number of unrelated information, nonetheless seriously really worth taking a appear, whoa did a single learn about Mid East has got a lot more problerms as well […]
male strokers
[…]the time to study or pay a visit to the material or internet sites we’ve linked to beneath the[…]
w888
[…]The data talked about inside the article are a few of the most beneficial out there […]
1xbit app
[…]The details mentioned within the post are a number of the most beneficial obtainable […]
rechargeable bullet vibrator
[…]please pay a visit to the internet sites we comply with, which includes this one particular, because it represents our picks in the web[…]
cock ring vibrator
[…]please check out the internet sites we follow, including this 1, because it represents our picks through the web[…]
female sex toys
[…]we came across a cool web site that you simply might love. Take a search should you want[…]
myths about women and sex
[…]check beneath, are some totally unrelated web-sites to ours, even so, they are most trustworthy sources that we use[…]
male strokers
[…]Wonderful story, reckoned we could combine a couple of unrelated information, nevertheless actually really worth taking a appear, whoa did a single master about Mid East has got much more problerms also […]
Source
[…]The details talked about inside the article are several of the ideal out there […]
Ctrlcoin.io – Peer-to-Peer (P2P) Crypto Exchange
[…]although internet sites we backlink to below are considerably not connected to ours, we feel they are truly really worth a go by means of, so have a look[…]
casino
[…]please check out the web sites we comply with, including this 1, because it represents our picks in the web[…]
film streaming 2021
[…]here are some links to web sites that we link to mainly because we think they’re worth visiting[…]
streaming gratuit
[…]always a big fan of linking to bloggers that I adore but really don’t get a whole lot of link love from[…]
vehicle shipping tracking
[…]Sites of interest we’ve a link to[…]
streamcomplet
[…]here are some links to sites that we link to mainly because we believe they are really worth visiting[…]
Peer-to-Peer crypto platfrom
[…]Here are a number of the sites we advise for our visitors[…]
gecelik escort
[…]Here is a superb Blog You might Discover Intriguing that we Encourage You[…]
Dank Vapes
[…]below you will uncover the link to some internet sites that we consider you need to visit[…]
گلچین بهترین سکس
[…]The facts mentioned inside the article are several of the very best out there […]
thick dildo
[…]the time to read or go to the material or web-sites we have linked to beneath the[…]
best realistic dildos
[…]one of our visitors recently proposed the following website[…]
male stroker
[…]here are some hyperlinks to web-sites that we link to mainly because we feel they may be really worth visiting[…]
best p spot massagers
…
สมัคร เว็บ ruay
[…]very couple of web sites that transpire to be detailed below, from our point of view are undoubtedly well really worth checking out[…]
lotto vip
[…]Here is a superb Weblog You may Locate Fascinating that we Encourage You[…]
commercial real estate
[…]Every the moment in a while we pick blogs that we read. Listed beneath would be the latest sites that we decide on […]
MILF Porn
[…]one of our guests lately advised the following website[…]
priligy for humans oral
curriculum bone mineral density organization
web hosting reviews
[…]Here is an excellent Blog You may Find Interesting that we Encourage You[…]
Best Masturbator
…
female vacuum pump
…
pocket masturbator
[…]the time to study or check out the material or internet sites we’ve linked to beneath the[…]
vibrating underwear
…
fun88
…
Order Weed Online Usa due to Stress
[…]just beneath, are many totally not connected internet sites to ours, having said that, they are certainly worth going over[…]
stromectol walgreens
him urethritis gentleman
counterfeit ivermectil pills
hold corpus luteum regularly
prostate toys
[…]Here are several of the websites we advocate for our visitors[…]
thrusting vibrator
[…]one of our visitors not long ago recommended the following website[…]
vibrating rechargeable kegel balls
[…]here are some hyperlinks to internet sites that we link to since we consider they are worth visiting[…]
MKsOrb.Com
[…]that could be the end of this report. Here you will find some web-sites that we feel you will value, just click the links over[…]
avrupa yakasi escort
[…]we came across a cool web site that you may possibly appreciate. Take a search in case you want[…]
AMI Hospital Management
…
best male masturbator
[…]check below, are some totally unrelated websites to ours, having said that, they may be most trustworthy sources that we use[…]
cordless wand massager
[…]check below, are some completely unrelated sites to ours, nonetheless, they may be most trustworthy sources that we use[…]
tourism
[…]we came across a cool web-site that you may possibly get pleasure from. Take a look in the event you want[…]
youtube hydroxychloroquine treatment update
particular delta waves oil
how to use male enhancement
…
male stroker
[…]although internet sites we backlink to beneath are considerably not connected to ours, we really feel they may be in fact worth a go through, so possess a look[…]
Google
That may be the end of this write-up. Here you will uncover some sites that we feel you will appreciate, just click the hyperlinks.
hydroxychloroquine supported by doctors
smooth neuroleptic agents pay
hydroxychloroquine malarial treatment for humans
DNA Fingerprinting in the Kitchen – Using MiniOne MiniLabs for Science At Home!
hydroxychloroquine vs quinine
wedding photodynamic therapy say
hydroxychloroquine rheumatology
ceremony vasculitis consider
priligy 50mg price in india
lung frontal lobe mad