Electrophoresis 101 MiniLab
One of the most popular MiniLabs with teachers, Electrophoresis 101 is a comprehensive introduction to the theory and practice of electrophoresis in an affordable, accessible package. Students pour, load, and run their own gels, observing the separation of dyes and DNA bands in real-time, and then plot a standard curve of size vs. migration distance to determine the sizes of unknown DNA fragments. In a single classroom period your students will gain the experience necessary to fully engage with and understand more advanced gel electrophoresis labs.
Introduce your students to powerful concepts in DNA analysis. Students cast, load, and run their own gels, watching dyes and DNA separate in real time. Challenge your students to determine the size of unknown pieces of DNA by creating a standard curve migration distances of known fragments. This guide provides sample answers to questions and explains certain steps in the protocol.