Restriction Analysis of DNA MiniLab


Challenge your students to predict the results of single and double digests, set up the digest reactions, and analyze the results with gel electrophoresis. This AP level restriction digestion lab provides the complete, hands-on experience with clear, clean, and reliable results. Recommended for AP, honors, and advanced biology students.

SKU: M6051 Category:


Molecular mechanisms for cutting DNA or RNA can help a cell ensure its safety and future, whether it’s for genetic recombination or in the case of bacteria, using restriction enzymes as a defense mechanism, slicing apart the DNA of a foreign invader. These molecular scissors allow researchers to study smaller pieces of DNA more thoroughly. In this restriction digestion lab, students will explore what restriction enzymes do, perform a single and double digest of DNA, predict fragment sizes and compare to fragment size of the actual digested DNA run on an agarose gel.

  • Teacher and student guides with background information, step-by-step procedures, questions for critical thinking, student worksheets for analysis, and sample answers for teachers
  • Hands-on lab that can be completed in two 50-minute class periods
  • Store at 4°C; GreenGel™ Cups should be left in the original box and protected from light
  • Guaranteed stable for three months with proper storage

Materials Included in Each MiniLab:

Each MiniLab contains enough materials for 10 workstations, 2 – 3 students per workstation.

Materials include:

  • Ten 1.5% agarose GreenGel™ Cups
  • Uncut DNA Sample
  • MiniOne® Universal DNA marker
  • Enzyme reaction buffer
  • Two restriction enzymes
  • MiniOne® 5X Sample Loading Dye
  • One bottle of 100 mL Tris-Borate-EDTA (TBE) buffer concentrate
  • One bag of 0.65 mL microcentrifuge tubes
  • One bag of 1 – 200 µL micropipette tips
  • Teacher guide and student worksheets

Teaching Resources

Utilize these teachers guides and classroom handouts to get the most out of your restriction digestion MiniLab.

* Teachers, please contact us for password to access this file.

Additional information

Weight 2 lbs
Dimensions 4.5 x 9 x 4.5 in

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