Molecular mechanisms for cutting DNA or RNA can help a cell ensure its safety and future, whether it’s for genetic recombination or in the case of bacteria, using restriction enzymes as a defense mechanism, slicing apart the DNA of a foreign invader. These molecular scissors allow researchers to study smaller pieces of DNA more thoroughly. In this restriction digestion lab, students will explore what restriction enzymes do, perform a single and double digest of DNA, predict fragment sizes and compare to fragment size of the actual digested DNA run on an agarose gel.
- Teacher and student guides with background information, step-by-step procedures, questions for critical thinking, student worksheets for analysis, and sample answers for teachers
- Hands-on lab that can be completed in two 50-minute class periods
- Store at 4°C; GreenGel™ Cups should be left in the original box and protected from light
- Guaranteed stable for three months with proper storage
Materials Included in Each MiniLab:
Each MiniLab contains enough materials for 10 workstations, 2 – 3 students per workstation.
- Ten 1.5% agarose GreenGel™ Cups
- Uncut DNA Sample
- MiniOne® Universal DNA marker
- Enzyme reaction buffer
- Two restriction enzymes
- MiniOne® 5X Sample Loading Dye
- One bottle of 100 mL Tris-Borate-EDTA (TBE) buffer concentrate
- One bag of 0.65 mL microcentrifuge tubes
- One bag of 1 – 200 µL micropipette tips
- Teacher guide and student worksheets
Utilize these teachers guides and classroom handouts to get the most out of your restriction digestion MiniLab.
* Teachers, please contact us for password to access this file.