In this engaging breast cancer genetics lab activity, students will delve into the world of breast cancer markers, exploring both the scientific and bioethical considerations associated with genetic testing. Armed with DNA electrophoresis, students will analyze specific genetic markers linked to breast cancer susceptibility. They will investigate the significance of these markers in assessing an individual’s risk of developing the disease and discuss the ethical implications surrounding the use of such information.
In this lab students will perform DNA electrophoresis and use a standard curve to determine the fragment sizes in order to identify the suspected marker involved in breast cancer.
Ideal for high school students, especially those in a biomedical science program, and can be used in the PLTW biomedical pathway.
Materials Included in Each MiniLab:
Each MiniLab contains enough materials for 10 workstations, 2 – 3 students per workstation.
- Four Ready-to-Load DNA samples
- MiniOne® Marker
- Ten 1.2% agarose GreenGel™ GelCups
- Two 50 mL BufferCups Tris-Borate-EDTA (TBE) buffer concentrate
- One bag of 0.65 mL microcentrifuge tubes
- One bag of 2 – 200 µL micropipette tips