Description
Did an influenzer just go viral??
A routine bus trip to an away game and suddenly your muscles ache, you have a fever, headache, and feel nauseous. Your doctor alerts you to the recent news about a fast moving flu and the need to test students and chaperones to prevent an outbreak in your hometown.
This MiniLab is based on the Influenza Outbreak Investigation activity from the Biotech Program at Shoreline Community College in Shoreline, WA. Dr. Jan Chalupny and Dr. Dina Kovarik have created extensive curricular materials as a part of a broader immunology unit. The link to these resources can be found in the teacher’s guide.
This influenza lab is ideal for advanced high school students and beginning college students in biology and biomedical programs.
Materials Included in Each MiniLab:
Each MiniLab contains enough materials for 10 workstations, 2 – 3 students per workstation.
- Ten 1.5% agarose GreenGel™ Cups
- One bottle of 100 mL Tris-Borate-EDTA (TBE) buffer concentrate, enough to make 2L of 1X running buffer
- FastTaq PCR MasterMix (2X)
- One primer set, forward and reverse
- Patient viral DNA samples (3 levels)
- Positive and negative DNA samples
- MiniOne® DNA marker
- MiniOne® 5X sample loading dye
- One bag of 0.2 mL thin-wall PCR tubes
- One bag of 0.65 mL microcentrifuge tubes
**Please note the following equipment is REQUIRED but not included:
- The MiniOne Electrophoresis System
- MiniOne PCR System with power supply + cord
- 2-200 uL pipette tips
The following equipment is suggested but not required:
- 2-200 ul micropipette
- Centrifuge capable of spinning PCR tubes at a minimum of 8000 RPM, such as the MiniOne Multi-Speed Centrifuge
