Pipetting is a fundamental and ubiquitous skill used in the molecular biology lab where samples volumes can be very small and possibly even scarce. Learning how to use a pipette includes determining what volume range your pipette can dispense, dialing to the correct volume, dispensing into the correct location and ensuring you are transferring the correct amount of liquid.
Dilutions are also another key technique since lab reagents are often provided as a concentrated solution that need to be diluted to a working concentration. In developing new products, protocols require optimization to understand which dilution factor and concentration provides the best results.
This introductory kit will give students the opportunity to learn how to use a variable volume pipette and develop the background to be able to load a gel. It will also provide the experience to calculate different concentrations and understand the differences between a direct and serial dilution. Dilution results are visualized on The Winston Fluorescence Reader giving the students an opportunity to see how fluorescence detection works.
Materials Included in each Bundle:
- Ten (10) M1050 MiniOne Winston Fluorescence Readers
- Ten (10) M2008 2-20 uL p20 adjustable volume micropipettes
- Ten (10) 1 mL transfer pipettes
- One (1) M3002 Gel Loading Practice MiniLab
- One (1) M3013 The Dilution Solution MiniLab
- Teacher’s guide for Gel Loading Practice MiniLab
- Teacher’s guide for Dilution Solution MiniLab
Utilize this teacher guide to make the most of your Pipetting Practice and Dilution Solution Introductory Kit: