Huntington’s disease (HD) is an inherited progressive, neurodegenerative disorder that is caused when the neurons (nerve cells) in the brain die. Signs and symptoms of Huntington’s disease include uncontrolled movement, memory loss, mood and behavior disturbances, and deteriorating cognitive (thinking) ability. Most people with Huntington’s disease start to display symptoms typically between 30 and 50 years of age, although there is also a less common form of the disease known as the juvenile form that begins in childhood or adolescence. There is currently no cure for Huntington’s disease but there are medications available to help manage the symptoms.
In this lab, students will examine family history to construct a pedigree and will assess molecular data to make predictions about inheritance of the disease in fraternal twins. Students will then perform gel electrophoresis to confirm genotype.
Appropriate for high school life science, genetics classes and AP Biology.
Materials Included in each MiniLab:
Each MiniLab contains enough materials for 10 workstations, 2 – 3 students per workstation.
- 5 Ready-to-load DNA samples: (4 samples, 1 DNA ladder)
- Ten 2% Agarose gel cups
- One bottle of 100 mL Tris-Borate-EDTA (TBE) buffer concentrate
- 0.65 mL microcentrifuge tubes: 11 tubes in 5 colors (55 total)
- One bag of 1 – 200 µL micropipette tips
- Teacher guide and student worksheets.
Utilize these teachers guides and classroom handouts to get the most out of your Hunt for Huntington’s MiniLab
- Teacher’s Guide *
- Student Guide
- Pedigree frame (Print at “Actual Size” on 11X17 size paper)
- Model pieces (Printat “Actual Size” on 8.5X11 size paper)
* Teachers, please contact us for password to access this file.