A Taste of Genetics MiniLab with Electrophoresis Reagents (M6010)

$115.00

Genetics gets personal as students extract and amplify their own DNA, then utilize PCR and electrophoresis to determine their genotype for the PTC tasting trait using a restriction digest assay.

 

Description

Bring polymerase chain reaction (PCR) and electrophoresis into your classroom with the Taste of Genetics PTC MiniLab! This fun and engaging Mendelian genetics lab allows students to identify their phenotype for bitter taste and investigate their genotype for the bitter taste gene, TAS2R38. Students will extract their own DNA, amplify a region of interest of the gene, use a restriction digest assay to cut the DNA, then separate and visualize the fragments using electrophoresis to determine their genetic profile of the of their TAS2R38 alleles.

  • Teacher and student guides with background information, step-by-step procedures, questions for critical thinking, student worksheets for analysis, and sample answers for teachers
  • Hands-on lab that can be completed in three 50-minute class periods
  • Store at 4°C; GreenGel™ Cups should be left in the original box and protected from light
  • Guaranteed stable for three months with proper storage

Teaching AP Biology? A Taste of Genetics MiniLab and extension activities are three Big Ideas in one comprehensive package. Together, these labs are an in-depth exploration of the TAS2R38 gene, covering hands-on genetic analysis, bioinformatics, population genetics, and evolution.

Materials Included in each MiniLab:

Each MiniLab contains enough materials for 10 workstations, 2 – 3 students per workstation.

Materials include:

  • Ten 2% Agarose GreenGel™ Cups
  • One bottle of 100 mL TBE buffer concentrate
  • DNA extraction solution
  • Forward and reverse primers for PTC genes
  • Taq polymerase master mix (2X)
  • HaeIII restriction enzyme
  • Restriction enzyme dilution buffer
  • MiniOne® Sample Loading Dye (5X)
  • MiniOne® Molecular Weight Marker
  • One bag of 0.2mL thin-walled PCR tubes
  • One bag of 0.65 mL microcentrifuge tubes
  • Forty PTC taste strips
  • Teacher guide and student worksheets

Teaching Resources

Use these teachers guides and classroom handouts to get the most out of your A Taste of Genetics MiniLab.

* Teachers, please contact us for password to access this file.

Additional information

Weight 2 lbs
Dimensions 4.5 x 9 x 4.5 in
"A Taste of Genetics" Reagents Only

Kit includes supplies for 10 student groups: reagents for DNA extraction, PCR amplification, PTC taste strips, PCR and microcentrifuge tubes.

"A Taste of Genetics" and Gel Electrophoresis Combo, with Micropipette Tips

Kit includes supplies for 10 student groups: reagents for DNA extraction, PCR, and electrophoresis gels & buffer, PTC taste strips, PCR and microcentrifuge tubes; ten racks of micropipette tips

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