If you needed a short piece of string but all you had was a long one, what would you do? You would probably grab a pair of scissors and cut it to the length you needed. In a somewhat similar fashion, cells have mechanisms for cutting long strands of nucleic acid into shorter strands- a type of molecular scissors. There are several reasons why cells need to cut their DNA or RNA. In this lab students will explore what restriction enzymes do and determine electrophoresis fragment sizes by comparing bands to the molecular weight standards.
- Teacher and student guides with background information, step-by-step procedures, questions for critical thinking, student worksheets for analysis, and sample answers for teachers
- Hands-on lab that can be completed in one 50-minute class period
- Store at 4°C; GreenGel™ Cups should be left in the original box and protected from light
- Guaranteed stable for six months with proper storage
- Appropriate for high school students (grades 9-12).
Materials Included in Each MiniLab:
Each MiniLab contains enough materials for 10 workstations, 2 – 3 students per workstation.
- Ten 1.5% agarose GreenGel™ Cups
- Four DNA Samples (three pre-digested, one undigested)
- MiniOne® Universal DNA marker
- One bottle of 100 mL Tris-Borate-EDTA (TBE) buffer concentrate, enough to make 2L of 1X running buffer
- One bag of 0.65 mL microcentrifuge tubes
- One bag of 2 – 200 µL micropipette tips
- Teacher’s guide
Utilize these teachers guides and classroom handouts to get the most out of your restriction digest MiniLab.
* Teachers, please contact us for password to access this file.