Jillian is the only non-taster in 3 generations of tasters in her family! How did this happen? In this Mendelian inheritance lab, students will make claims about the bitter taste genotypes of Jillian’s family based on her phenotype as a non-taster, use electrophoresis to test their hypotheses, and construct a Log-Y standard curve to determine the fragment sizes to confirm the fragments are the sizes expected for the 3 different genotypes.
The entire lab can be completed in the span of a single classroom period, and helps teach concepts of inheritance, genetics, physiology, and fragment size analysis using standard curves. Like all our MiniLabs, it allows students to fully engage with the experimental process, from generating a hypothesis, to pouring, loading, and running agarose gels, and analyzing the results. Minimal prep work and clean up give teachers the time they need for lecture and discussion.
Ideal for high school students taking AP Biology.
Materials Included in Each MiniLab:
Each reagent pack contains enough materials for 10 workstations, 2 – 3 students per workstation.
- Ten 2% agarose GreenGel™ Cups
- Six pre-digested DNA samples
- MiniOne® DNA Marker
- Forty pieces of PTC tasting and taste control papers
- One bottle of 100 mL Tris-Borate-EDTA
- (TBE) buffer concentrate
- One bag of 0.65 mL microcentrifuge tubes
- One bag of 2–200 μL micropipette tips
- Teacher’s Guide