Description
Explain biological phenomena at a molecular level. The AP Bio Fundamentals Expanded Single User Reagent Set is about solving genetic puzzles.
First practice pipetting with the Gel Loading Practice Single User Reagent Set. Next learn about how restriction enzymes work by running DNA samples with single cuts and a double cut, and evaluate the results on a gel.
Using this foundation, see how restriction enzymes can help identify a single nucleotide polymorphism that can be used to determine the difference between two alleles for the bitter taste. The different alleles can be visualized by electrophoresis, which can be used to help explain how Jillian became a non-taster in her family of tasters. Also, learn how to use a standard curve to determine the fragment sizes of the resulting DNA bands.
Then take a look at a Huntington’s Disease case study, and help piece together this genetic puzzle to build out a family tree, learn about the unique ways HD is inherited, and help a couple understand the risks their kids face of inheriting this deadly disease.
Finally try to crack the mystery of the Foodborne Illness by reviewing interview data of party attendees, some who got sick and others who did not. Once you’ve discussed what likely made people sick, design an activity to whittle down and confirm which ingredient went bad.
The single user reagent sets are pre-aliquoted for an individual workstation making it easy for you to open the pack and start your electrophoresis with even less prep, and include one bag of 2 – 200 µL micropipette tips. These activities require a MiniOne Electrophoresis System with p20 pipette (not included).
Single User Reagent Set Materials included for ONE workstation:
1 Gel Loading Practice Single User Reagent Set
- One Gel Loading Practice Agar Disk with Preformed Wells
- One Pipette Target
- One set of dye samples
1 Restriction Digest Basics Single User Reagent Set
- Five DNA samples (three pre-digested, one undigested, MiniOne Marker)
- One 1.5% agarose GreenGel™ Cup
- One backup set of agarose gel cubes
- One tube of TBE Buffer, enough to make 135 mL of 1X running buffer
1 PTC Inheritance and Graphical Analysis Single User Reagent Set
- Six DNA samples (5 family members plus MiniOne DNA Marker)
- One each PTC Taste strip and taste control paper
- One 2% agarose GreenGel™ Cup
- One backup set of agarose gel cubes
- One tube of TBE Buffer, enough to make 135 mL of 1X running buffer
1 Huntington’s Disease Single User Reagent Set
- Five DNA samples (Four family members MiniOne 100bp Marker)
- One 2% agarose GreenGel™ Cup
- One backup set of agarose gel cubes
- One tube of TBE Buffer, enough to make 135 mL of 1X running buffer
1 Foodborne Outbreak Single User Reagent Set
- Eleven DNA samples (6 food samples, 2 markers, 3 standards/controls)
- One 1% agarose GreenGel™ Cup
- One backup set of agarose gel cubes
- One tube of TBE Buffer, enough to make 135 mL of 1X running buffer
Also included: one bag of 2 – 200 µL micropipette tips
